Glassy carbon electrodes sequentially modified by cysteamine-capped gold nanoparticles and poly(amidoamine) dendrimers generation 4.5 for detecting uric acid in human serum without ascorbic acid interference

•We constructed a modified electrode for detecting uric acid contained in human serum.•Electrochemical detection of uric acid was comparable with the enzymatic method.•Interference of ascorbic acid was not observed. Glassy carbon electrodes (GCE) were sequentially modified by cysteamine-capped gold...

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Veröffentlicht in:	Analytica chimica acta 2014-02, Vol.812, p.18-25
Hauptverfasser:	Ramírez-Segovia, A.S., Banda-Alemán, J.A., Gutiérrez-Granados, S., Rodríguez, A., Rodríguez, F.J., Godínez, Luis A., Bustos, E., Manríquez, J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Ascorbic Acid - chemistry Carbon - chemistry Cysteamine - chemistry Dendrimers Electrodes Glassy carbon Gold Gold - chemistry Gold nanoparticles Human Human serum Metal Nanoparticles Modified electrodes Nanoparticles Polyamines - chemistry Serums Uric acid Uric Acid - blood
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Zusammenfassung:	•We constructed a modified electrode for detecting uric acid contained in human serum.•Electrochemical detection of uric acid was comparable with the enzymatic method.•Interference of ascorbic acid was not observed. Glassy carbon electrodes (GCE) were sequentially modified by cysteamine-capped gold nanoparticles (AuNp@cysteamine) and PAMAM dendrimers generation 4.5 bearing 128-COOH peripheral groups (GCE/AuNp@cysteamine/PAMAM), in order to explore their capabilities as electrochemical detectors of uric acid (UA) in human serum samples at pH 2. The results showed that concentrations of UA detected by cyclic voltammetry with GCE/AuNp@cysteamine/PAMAM were comparable (deviation
ISSN:	0003-2670 1873-4324
DOI:	10.1016/j.aca.2013.12.025