Mechanochemical Stimulation of MCF7 Cells with Rod-Shaped Fe-Au Janus Particles Induces Cell Death Through Paradoxical Hyperactivation of ERK

Multifunctional nanoparticles that actively target‐specific tissues are studied for cancer diagnosis and treatment. Magnetically and optically active particles are of particular interest because they enable multiple imaging modalities and physically modulated therapies, such as magnetic hyperthermia. Fe–Au nanorods are synthesized that have a long iron segment, coated with polyethylene glycol, and a short gold tip functionalized with heregulin (HRG), a known ligand of ErbB family of receptors. HRG–nanorods preferentially target MCF7 cells relative to MDA‐MB‐231 cells, as demonstrated in a novel microfluidics device. Targeting rates of these classical breast cancer cells correlate with their differential expression of ErbB2/3 receptors. HRG–nanorod binding stimulates the extracellular signal‐regulated kinase 1/2 (ERK) phosphorylation in MCF7 cells. The increase in ERK phosphorylation is linked to “active zones,” dynamic regions in the cell periphery, which exhibit higher rates of particle binding than the rest of the cell. Periodically stretching cells using magnetic tweezers further activates ERK, which leads to cell death in cells co‐treated with B‐Raf inhibitors, through ERK hyperactivation. Although to a lesser extent, cell death is also achieved through magnetic hyperthermia. These results demonstrate nanoscale targeting and localized mechanochemical treatment of specific cancer cell lines based on their receptor expression using multifunctional nanoparticles. Iron nanorods with heregulin‐functionalized gold tips preferentially target MCF7 cells compared with MDA‐MB‐231 cells where targeting rates correlate relative ErbB3 receptor expression levels. Nanorod binding induces localized ERK phosphorylation, which is pronounced when cells are periodically stretched. Co‐treatment with B‐Raf inhibitors results in cell death through ERK hyperstimulation, demonstrating the nanoscale targeting and localized mechanochemical treatment of specific cancer cells.