Bioprospecting of fungi associated with marine biota from Kepulauan Seribu as Antitumor T47D and HepG2

Marine fungi has become a promising source in the search for new antitumor compounds. This study aims to isolate marine fungi associated with sponges and soft corals from Kepulauan Seribu Marine National Park and to examine the prospects of these isolates as producers of anti breast-tumor T47D and anti liver-tumor HepG2 agents. A number of 46 isolates of fungi were isolated from 17 samples of sponges and soft corals using isolation medium of Malt Extract Agar (MEA), Glucose Peptone Yeast (GPY) and Minimal Fungal Medium (MFM). Isolates of the fungi were cultured in liquid medium (100 mL) for 4 weeks at room temperature (27-28 degree C) in static conditions. Crude extract of each isolate was then tested against breast tumor cells (T47D) and liver tumor cells (HepG2) at a concentration of 30 mu g/mL using MTT assay method. There were 6 isolates of the fungi that considered active as anti breast-tumor (T47D), i.e. MFP 42 (42.28%), MFP 48 (52.36%), MFP 49 (44.83%), MFP 59 (47.5%), MFP 60 (44.12%), MFP 65 (41.27%), MFP 70 (40.21%) and 1 isolate (MFP 64) was active as both anti breast-tumor T47D (43.85%) and anti liver-tumor HepG2 (40.22%).Original Abstract: Kapang laut menjadi sumber yang menjanjikan dalam pencarian senyawa antitumor baru. Penelitian ini bertujuan untuk mengisolasi kapang yang berasosiasi dengan spons dan karang lunak asal Taman Nasional Laut Kepulauan Seribu dan menguji prospek isolat-isolat tersebut sebagai penghasil senyawa bioaktif antitumor payudara (T47D) dan antitumor liver (HepG2). Sejumlah 46 isolat kapang telah diisolasi dari 17 sampel spons dan karang lunak dengan menggunakan media isolasi Malt Extract Agar (MEA), Glucose Peptone Yeast (GPY) dan Minimal Fungal Medium (MFM). Masing-masing isolat kapang dikultur dalam media cair (100 mL) selama 4 minggu pada suhu ruang (27-28 degree C) dalam kondisi statis. Ekstrak kasar dari tiap isolat kapang selanjutnya diuji aktivitas sitotoksiknya terhadap sel tumor payudara (T47D) dan sel tumor liver (HepG2) pada konsentrasi 30 mu g/mL dengan metode uji MTT. Terdapat 6 isolat kapang yang aktif sebagai antitumor payudara (T47D) , yaitu isolat MFP 42 (42,28%), MFP 48 (52,36%), MFP 49 (44,83%), MFP 59 (47,5%), MFP 60 (44,12%), MFP 65 (41,27%), MFP 70 (40,21%) dan terdapat 1 isolat kapang (MFP 64) yang selain aktif sebagai antitumor payudara/T47D (43,85%) juga aktif sebagai antitumor liver/HepG2 (40,22%).