DEVELOPMENT OF ANALYTICAL TECHNIQUES FOR IDENTIFICATION OF PHYTOCHEMICALS IN SELECTED HERBAL DRUGS

The present work attempted to identify and standardize the different phytochemical by modern analytical techniques and to summarize the UV, TLC, FTIR characters of the plants; Eugenia caryophyllus (clove), Acorus Calamus (vekhand), Myristica fragrans (Nutmeg) and Citrus aurantium(Orange peel) which ultimately results in compilation of analytical data for these crude drugs which are very much used in herbal cosmetics. The methanolic extracts of Clove, Acorus, Nutmeg, Orange peel were prepared by ultrasonic bath extraction method and labeled as DSC, DSA, DSN and DSO respectively for further studies. Preliminary pytochemical screening of extracts were also performed. Asarones were detected in Acorus extract by subjecting the TLC to UV light and observing blue fluorescence. Volatile oil was isolated from clove buds by using Clevenger's apparatus which was subjected for the FTIR analysis, which indicates the peak for presence of phenol compound. For nutmeg and orange peel samples the presence of myristicine, elemicine in nutmeg extract was confirmed by Rf values.