Comparison of Hepatotoxicity of 1,2-, 1,3- and 1,4-Dibromobenzenes: the Dynamics of Changes of Selected Parameters of Liver Necrosis in Acute Poisoning in Mice

Various doses of dibromobenzene isomers (1,2‐dBB, 1,3‐dBB, 1,4‐dBB) were administered (i.p.) to BALB mice. The levels of reduced glutathione (GSH) and malondialdehyde (MDA) in the liver, and glutamate–pyruvate transaminase (GPT) (EC.2.6.1.2) γ‐glutamyltransferase (γ‐GT) (EC.2.3.2.2) and triglycerides (TG) in the serum were estimated. A considerable decrease of GSH was observed between 2 and 12 h after administration of the compounds. Increases in serum GPT activity (up to 100‐fold) and γ‐GT (three‐to fivefold) were observed after treatment using 1,2‐ and 1,3‐dibromobenzenes; TG decreased in concentration initially and then slightly increased. Histopathological examination confirmed the strong necrotic effect of 1,2‐ and 1,3‐dBB isomers. No such changes (elevation of serum GPT activity and necrosis) were noticed after 1,4‐dBB.