Isolation of CD-binding protein of water hyacinth (Eichhornia crassipes) grown in Nile River water
Water hyacinths (Eichhornia Crassipes) collected from the Nile river were exposed to concentrations of 100 ppm cadmium. Solutions were renewed every 3 d. Plants were harvested and cadmium binding proteins isolated and purified with Sephadex A-25 and fractionated on Sephadex G100. Thirty five per cen...
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Veröffentlicht in: | Water, air, and soil pollution air, and soil pollution, 1996-01, Vol.87 (1/4), p.357-362 |
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creator | El-Enany, A.E Mazen, A.M.A |
description | Water hyacinths (Eichhornia Crassipes) collected from the Nile river were exposed to concentrations of 100 ppm cadmium. Solutions were renewed every 3 d. Plants were harvested and cadmium binding proteins isolated and purified with Sephadex A-25 and fractionated on Sephadex G100. Thirty five per cent of accumulated cadmium was associated with protein of molecular weight between 20-25 kD and 40 per cent with protein of molecular weight 8-12 kD. Both proteins were isolated from plants exposed to control levels of 0.03 ppm cadmium although accumulated cadmium concentrations were lower. |
doi_str_mv | 10.1007/BF00696847 |
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Solutions were renewed every 3 d. Plants were harvested and cadmium binding proteins isolated and purified with Sephadex A-25 and fractionated on Sephadex G100. Thirty five per cent of accumulated cadmium was associated with protein of molecular weight between 20-25 kD and 40 per cent with protein of molecular weight 8-12 kD. Both proteins were isolated from plants exposed to control levels of 0.03 ppm cadmium although accumulated cadmium concentrations were lower.</description><identifier>ISSN: 0049-6979</identifier><identifier>EISSN: 1573-2932</identifier><identifier>DOI: 10.1007/BF00696847</identifier><identifier>CODEN: WAPLAC</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Animal, plant and microbial ecology ; Applied ecology ; binding ; binding proteins ; Biological and medical sciences ; Biological treatment of waters ; Biotechnology ; cadmium ; Ecotoxicology, biological effects of pollution ; Effects of pollution and side effects of pesticides on plants and fungi ; Eichhornia crassipes ; Environment and pollution ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Industrial applications and implications. 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Solutions were renewed every 3 d. Plants were harvested and cadmium binding proteins isolated and purified with Sephadex A-25 and fractionated on Sephadex G100. Thirty five per cent of accumulated cadmium was associated with protein of molecular weight between 20-25 kD and 40 per cent with protein of molecular weight 8-12 kD. Both proteins were isolated from plants exposed to control levels of 0.03 ppm cadmium although accumulated cadmium concentrations were lower.</description><subject>Animal, plant and microbial ecology</subject><subject>Applied ecology</subject><subject>binding</subject><subject>binding proteins</subject><subject>Biological and medical sciences</subject><subject>Biological treatment of waters</subject><subject>Biotechnology</subject><subject>cadmium</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Effects of pollution and side effects of pesticides on plants and fungi</subject><subject>Eichhornia crassipes</subject><subject>Environment and pollution</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>metal tolerance</subject><subject>plant proteins</subject><subject>purification</subject><subject>river water</subject><subject>water pollution</subject><subject>water purification</subject><issn>0049-6979</issn><issn>1573-2932</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkU9LAzEQxYMoWKsXv4A5iKiwmn-7SY5aWy0UBbXnJZsmbWTd1GRr6bc3tkWPncvAzO89ZngAnGJ0gxHit_cDhApZCMb3QAfnnGZEUrIPOggxmRWSy0NwFOMHSiUF74BqGH2tWucb6C3sPWSVayaumcJ58K1x6-lStSbA2Upp17QzeNl3ejbzoXEK6qBidHMTr-A0-GUDk-LZ1Qa-uu-kWSuPwYFVdTQn294F40H_vfeUjV4eh727UaYpz9us4FbjCrHKMKGtLYQRE1NxrSk1ghBpCTFa2Kqyk7THeYUUnyiDKWKK4kLQLrjY-KbTvxYmtuWni9rUtWqMX8QScyQ4ofluMOekkIztBmnBmKA8gdcbUAcfYzC2nAf3qcKqxKj8Dab8DybB51tXFbWqbVCNdvFPQRGVXMiEnW0wq3yppiEh4zeC0r84JxzniP4AhV-WXQ</recordid><startdate>19960101</startdate><enddate>19960101</enddate><creator>El-Enany, A.E</creator><creator>Mazen, A.M.A</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7TV</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>19960101</creationdate><title>Isolation of CD-binding protein of water hyacinth (Eichhornia crassipes) grown in Nile River water</title><author>El-Enany, A.E ; Mazen, A.M.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-67fc1b04be48cff68e8deb7cc33e8229f22ec8fbbfd8cf15b0a7dae1304a31683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animal, plant and microbial ecology</topic><topic>Applied ecology</topic><topic>binding</topic><topic>binding proteins</topic><topic>Biological and medical sciences</topic><topic>Biological treatment of waters</topic><topic>Biotechnology</topic><topic>cadmium</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Effects of pollution and side effects of pesticides on plants and fungi</topic><topic>Eichhornia crassipes</topic><topic>Environment and pollution</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>metal tolerance</topic><topic>plant proteins</topic><topic>purification</topic><topic>river water</topic><topic>water pollution</topic><topic>water purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El-Enany, A.E</creatorcontrib><creatorcontrib>Mazen, A.M.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Pollution Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Water, air, and soil pollution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El-Enany, A.E</au><au>Mazen, A.M.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of CD-binding protein of water hyacinth (Eichhornia crassipes) grown in Nile River water</atitle><jtitle>Water, air, and soil pollution</jtitle><date>1996-01-01</date><risdate>1996</risdate><volume>87</volume><issue>1/4</issue><spage>357</spage><epage>362</epage><pages>357-362</pages><issn>0049-6979</issn><eissn>1573-2932</eissn><coden>WAPLAC</coden><abstract>Water hyacinths (Eichhornia Crassipes) collected from the Nile river were exposed to concentrations of 100 ppm cadmium. Solutions were renewed every 3 d. Plants were harvested and cadmium binding proteins isolated and purified with Sephadex A-25 and fractionated on Sephadex G100. Thirty five per cent of accumulated cadmium was associated with protein of molecular weight between 20-25 kD and 40 per cent with protein of molecular weight 8-12 kD. Both proteins were isolated from plants exposed to control levels of 0.03 ppm cadmium although accumulated cadmium concentrations were lower.</abstract><cop>Dordrecht</cop><pub>Springer</pub><doi>10.1007/BF00696847</doi><tpages>6</tpages></addata></record> |
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ispartof | Water, air, and soil pollution, 1996-01, Vol.87 (1/4), p.357-362 |
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source | Springer Nature - Complete Springer Journals |
subjects | Animal, plant and microbial ecology Applied ecology binding binding proteins Biological and medical sciences Biological treatment of waters Biotechnology cadmium Ecotoxicology, biological effects of pollution Effects of pollution and side effects of pesticides on plants and fungi Eichhornia crassipes Environment and pollution Freshwater Fundamental and applied biological sciences. Psychology Industrial applications and implications. Economical aspects metal tolerance plant proteins purification river water water pollution water purification |
title | Isolation of CD-binding protein of water hyacinth (Eichhornia crassipes) grown in Nile River water |
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