Isolation of CD-binding protein of water hyacinth (Eichhornia crassipes) grown in Nile River water

Water hyacinths (Eichhornia Crassipes) collected from the Nile river were exposed to concentrations of 100 ppm cadmium. Solutions were renewed every 3 d. Plants were harvested and cadmium binding proteins isolated and purified with Sephadex A-25 and fractionated on Sephadex G100. Thirty five per cent of accumulated cadmium was associated with protein of molecular weight between 20-25 kD and 40 per cent with protein of molecular weight 8-12 kD. Both proteins were isolated from plants exposed to control levels of 0.03 ppm cadmium although accumulated cadmium concentrations were lower.