Cloning of a Bacillus sp. BP-23 gene encoding a xylanase with high activity against aryl xylosides

The xynB gene encoding a xylanase from the recently isolated Bacillus sp. strain BP-23 has been cloned and expressed in Escherichia coli. The enzyme produced in this host shows a molecular size of 41 kDa and a pI of 4.5. The pH and temperature at which the highest activity was found were 5.5 and 50...

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Veröffentlicht in:	FEMS microbiology letters 1996-04, Vol.137 (2/3), p.285-290
Hauptverfasser:	Blanco, A, Diaz, P, Martinez, J, Lopez, O, Soler, C, Pastor, F.I.J
Format:	Artikel
Sprache:	eng
Schlagworte:	Aromatic compounds Aryl xyloside Bacillus Bacillus (bacteria) Bacillus - enzymology Bacillus - genetics Bacteriology Biodegradation, Environmental Biological and medical sciences Cloning Cloning, Molecular E coli enzyme activity Enzymes Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology gene expression Genes, Bacterial Genetics Glycosides - metabolism Hydrogen-Ion Concentration Isoelectric Point Microbiology Molecular Weight O-glycoside hydrolases oligosaccharides physicochemical properties Restriction Mapping structural genes Substrate Specificity Temperature Transxylosidase Xylan Xylan Endo-1,3-beta-Xylosidase Xylanase xylanase b xylanolytic microorganisms xylooligosaccharides Xylosidases - chemistry Xylosidases - genetics Xylosidases - metabolism XynB gene
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Beschreibung
Zusammenfassung:	The xynB gene encoding a xylanase from the recently isolated Bacillus sp. strain BP-23 has been cloned and expressed in Escherichia coli. The enzyme produced in this host shows a molecular size of 41 kDa and a pI of 4.5. The pH and temperature at which the highest activity was found were 5.5 and 50 degrees C respectively. Crude xylanase B showed activity on xylan, aryl xylosides, xylotetraose and xylotriose, while xylobiose was not hydrolyzed by the enzyme. Xylanase B showed high specific activity on aryl xylosides, probably as a result of the transxylosidase activity detected.
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.1996.tb08120.x