Ultrasensitive strategy based on PtPd nanodendrite/nano-flower-like@GO signal amplification for the detection of long non-coding RNA

Highly up-regulated in liver cancer (HULC) is a novel promising noninvasive biomarker for hepatocellular carcinoma (HCC), which is a kind of long non-coding RNAs (lncRNAs). But traditional methods limited HULC clinical detection for ownself drawbacks. Development a new HULC detection approach is urgent and necessary. Electrochemical nucleic acid sensor based on different signal amplification strategies with high sensitivity, fast, simple, and convenient, may solve this problem. Herein, we propose a novel strategy based on Pt–Pd bimetallic nanodendrites/nanoflower-like clusters on graphene oxide/Au/horseradish peroxidase (PtPd BND/BNF@GO/Au/HRP) to enhance the catalytic efficiency and sensitivity. And Au particles were simultaneously and separately capped with thionine or detection probe, which increase the binding amount of detection probe and decrease the electronic background. The results indicated that the catalytic effect was noticeably elevated and that the biosensor provides ultrasensitive detection for the lncRNA HULC. The linear calibration of the biosensor ranged from 1.00×10−3 to 1.00×103pM/mL, and the limit of detection was 0.247fM/mL. The lncRNA biosensor based on the PtPd BND/BNF@GO/Au/HRP/Au/thionine exhibited acceptable reproducibility and clear selectivity. This strategy may provide a new alternative for clinical HCC diagnosis through the detection of HULC. •Pt-Pd BND/BNF@GO stereochemical structure was synthesized to increase specific surface area and enhance catalyst capability.•Electrochemical biosensor was firstly applied to detect lncRNA HULC in HCC samples.•.A triple signal amplification strategy using the high catalytic activity of the Pt-Pd BND/BNF and HRP toward H2O2 was employed.•Au electrodeposition on SWCNTs was used as the sensor reaction platform.•Thionine and detection probe were synchronously and separately capped on Au particles to accelerate fabrication time and decrease electronic background.