The arc mutants of Arabidopsis with fewer large chloroplasts have a lower mesophyll conductance

Photosynthetic cells of most land plant lineages have numerous small chloroplasts even though most algae, and even the early diverging land plant group the hornworts, tend to have one or a few large chloroplasts. One constraint that small chloroplasts could improve is the resistance to CO 2 diffusion from the atmosphere to the chloroplast stroma. We examined the mesophyll conductance (inverse of the diffusion resistance) of mutant Arabidopsis thaliana plants with one or only a few large chloroplasts per cell. The accumulation and r eplication of c hloroplasts ( arc ) mutants of A. thaliana were studied by model fitting to gas exchange data and 13 CO 2 discrimination during carbon fixation. The two methods generally agreed, but the value of the CO 2 compensation point of Rubisco ( Γ * ) used in the model had a large impact on the estimated photosynthetic parameters, including mesophyll conductance. We found that having only a few large chloroplasts per cell resulted in a 25–50 % reduction in the mesophyll conductance at ambient CO 2 .