Glyptal as a support for enzyme immobilisation
Glyptal, a polyester obtained from phthalic anhydride and glycerol, was used as a support for protein immobilisation. Hydrazide groups were introduced in the polymer and then converted to azide groups, through which protein was covalently immobilised. Amyloglucosidase was used as a model and an inso...
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Veröffentlicht in: | Biotechnology techniques 1996-01, Vol.10 (1), p.59-62 |
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Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Glyptal, a polyester obtained from phthalic anhydride and glycerol, was used as a support for protein immobilisation. Hydrazide groups were introduced in the polymer and then converted to azide groups, through which protein was covalently immobilised. Amyloglucosidase was used as a model and an insoluble water derivative was synthesised retaining 24% of the specific activity of the native enzyme. Some properties of this immobilised enzyme were studied: Km (4.54 g.1 super(-1) using starch as substrate), optimal temperature (55 degree C) and half life (8 days). Furthermore, ferromagnetic-azide-glyptal derivative showed to be useful for the amyloglucosidase immobilisation. |
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ISSN: | 0951-208X 1573-6784 |
DOI: | 10.1007/BF00161085 |