Determination of amphetamines in biological samples using electro enhanced solid-phase microextraction-gas chromatography

•Ordered mesoporous carbon with uniform pore size was applied as a coating for EE-SPME.•The EE-SPME exhibits superior extraction efficiency for amphetamines over SPME.•The EE-SPME-GC method was used for the analysis of amphetamines in biological samples. In this work, an ordered mesoporous carbon (OMC)/Nafion coated fiber for solid-phase microextraction (SPME) was prepared and used as the working electrode for electro-enhanced SPME (EE-SPME) of amphetamines. The EE-SPME strategy is primarily based on the electro-migration and complementary charge interaction between fiber coating and ionic compounds. Compared with traditional SPME, EE-SPME exhibited excellent extraction efficiency for amphetamine (AP) and methamphetamine (MA) with an enhancement factor of 7.8 and 12.1, respectively. The present strategy exhibited good linearity for the determination of AP and MA in urine samples in the range of 10–1000ngmL−1 and 20–1000ngmL−1, respectively. The detection limits were found to be 1.2ngmL−1 for AP and 4.8ngmL−1 for MA. The relative standard deviations were calculated to be 6.2% and 8.5% for AP and MA, respectively. Moreover, the practical application of the proposed method was demonstrated by analyzing the amphetamines in urine and serum samples with satisfactory results.