Hybridoma perfusion system using a sedimentation device

A novel sedimentation method with a spiral decanter was utilized with a bioreactor for propagation of hybridoma cells at high densities. The live cell concentration was increased and cell lysis was greatly reduced in this system compared to a tangential flow hollow fiber perfusion system. The specif...

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Veröffentlicht in:	Biotechnology techniques 1995-11, Vol.9 (11), p.837-842
Hauptverfasser:	LAPORTE, T. L, SHEVITZ, J, KIM, Y, WANS, S. S
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal cells Biological and medical sciences Biotechnology Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies
Online-Zugang:	Volltext
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