Tip-enhanced Raman scattering—Targeting structure-specific surface characterization for biomedical samples

Tip-enhanced Raman scattering (TERS) has become a powerful tool for nanoscale structural analysis for several branches of organic, inorganic, and biological chemistry. This highly sensitive technique enables molecular characterization with a lateral resolution far beyond Abbe's diffraction limit and correlates structural and topographic information on a nanometer scale. In this review, the current experimental concepts with respect to their strengths and obstacles are introduced and discussed. A further focus was set to biochemistry comprising applications like nucleic acids, proteins, and microorganisms, thus demonstrating the potential use towards the pharmaceutically relevant challenges where nanometer resolution is required. [Display omitted]