Synthesis of the dendritic type β-cyclodextrin on primary face via click reaction applicable as drug nanocarrier

•Novel glycodendrimer from β-CD in core and branches was synthesized.•Supramolecular inclusion complex by dendrimer and MTX drug was prepared.•MTT assay show that the resultant dendrimer is not cytotoxic to the cells.•Results showed that the developed dendrimer could offer a controlled delivery of MTX. The objective of this study was the syntheses of well-defined glycodendrimer with entrapment efficiency by click reactions, with β-cyclodextrins (β-CDs) moiety to keep the biocompatibility properties, besides especially increase their capacity to load numerous appropriate sized guests. The original dendrimer containing β-CD in both periphery and central was synthesized using click reaction. The entrapment property of the β-CD-dendrimer was studied by methotrexate (MTX) drug. The chemical structure of β-CD-dendrimer was characterized by 1H NMR, 13C NMR and FTIR and its inclusion complex structure were investigated by SEM, DLS, DSC and FTIR techniques. The cytotoxic effect of obtained compound and its inclusion complex with MTX was analyzed using MTT test. The MTT test exhibited that the synthesized compound was not cytotoxic to the cell line considered. The in vitro drug release study turned out that the obtained β-CD dendrimer could be a suitable controlled drug delivery system for cancer treatment.