Green fluorescent protein production in the cellular slime molds Polysphondylium pallidum and Dictyostelium discoideum

The green fluorescent protein-encoding gene from Aequorea victoria has been cloned into several different transforming vectors and expressed in the cellular slime molds, Polysphondylium pallidum and Dictyostelium discoideum. We find that the protein is stable and non-toxic in both species, can be ea...

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Veröffentlicht in:	Gene 1995, Vol.165 (1), p.127-130
Hauptverfasser:	Fey, Petra, Compton, Kate, Cox, Edward C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aequorea victoria animal proteins Animals Base Sequence cloning coelenterata Dictyostelium Dictyostelium - genetics Dictyostelium - metabolism Dictyostelium discoideum FACS sorting Flow Cytometry fluorescence fungi gene expression gene transfer Gene Transfer Techniques genetic transformation Genetic Vectors GFP gfp gene Green Fluorescent Proteins Luminescent Proteins - genetics Luminescent Proteins - isolation & purification Luminescent Proteins - metabolism Molecular Sequence Data morphogenesis promoter regions recombinant DNA reporter genes structural genes transformation
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Beschreibung
Zusammenfassung:	The green fluorescent protein-encoding gene from Aequorea victoria has been cloned into several different transforming vectors and expressed in the cellular slime molds, Polysphondylium pallidum and Dictyostelium discoideum. We find that the protein is stable and non-toxic in both species, can be easily visualized in living and fixed specimens, and can be used to purify rare cells by fluorescence-activated cell sorting (FACS).
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(95)00430-E