ISOLATION AND CHARACTERIZATION OF A cDNA ENCODING INTERLEUKIN 2 FROM THE FLORIDA MANATEE, TRICHECHUS MANATUS LATIROSTRIS
Interleukin‐2 (IL‐2) production is essential for optimal mammalian immune response; therefore, a cDNA encoding IL‐2 was isolated and characterized for the endangered Florida manatee, Trichechus manatus latirostris. The manatee IL‐2 cDNA was cloned by reverse transcription/polymerase chain reaction (...
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Veröffentlicht in: | Marine mammal science 1996-01, Vol.12 (1), p.89-98 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Interleukin‐2 (IL‐2) production is essential for optimal mammalian immune response; therefore, a cDNA encoding IL‐2 was isolated and characterized for the endangered Florida manatee, Trichechus manatus latirostris. The manatee IL‐2 cDNA was cloned by reverse transcription/polymerase chain reaction (PCR), using primers derived from IL‐2 regions conserved among various mammalian species. Manatee IL‐2 cDNA consisted of 450 base pairs encoding a predicted product of 150 amino acid residues and displayed 87%, 82%, 79%, 78%, 75%, and 73% DNA sequence homology and 74%, 69%, 63%, 62%, 61%, and 57% amino acid sequence homology with human, pig, sheep, cow, rat, and mouse IL‐2, respectively. |
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ISSN: | 0824-0469 1748-7692 |
DOI: | 10.1111/j.1748-7692.1996.tb00306.x |