Embryonic Stem Cell Specific "Master" Replication Origins at the Heart of the Loss of Pluripotency: e1003969

Embryonic Stem Cell Specific "Master" Replication Origins at the Heart of the Loss of Pluripotency: e1003969

Epigenetic regulation of the replication program during mammalian cell differentiation remains poorly understood. We performed an integrative analysis of eleven genome-wide epigenetic profiles at 100 kb resolution of Mean Replication Timing (MRT) data in six human cell lines. Compared to the organiz...

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Veröffentlicht in:PLoS computational biology 2015-02, Vol.11 (2)
Hauptverfasser: Julienne, Hanna, Audit, Benjamin, Arneodo, Alain
Format: Artikel
Sprache:eng
Schlagworte:
Deoxyribonucleic acid
DNA
DNA methylation
Epigenetics
Gene expression
Genomes
Light
Standard deviation
Stem cells
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Zusammenfassung:Epigenetic regulation of the replication program during mammalian cell differentiation remains poorly understood. We performed an integrative analysis of eleven genome-wide epigenetic profiles at 100 kb resolution of Mean Replication Timing (MRT) data in six human cell lines. Compared to the organization in four chromatin states shared by the five somatic cell lines, embryonic stem cell (ESC) line H1 displays (i) a gene-poor but highly dynamic chromatin state (EC4) associated to histone variant H2AZ rather than a HP1-associated heterochromatin state (C4) and (ii) a mid-S accessible chromatin state with bivalent gene marks instead of a polycomb-repressed heterochromatin state. Plastic MRT regions ([
ISSN:1553-734X
1553-7358
DOI:10.1371/journal.pcbi.1003969