Identification of 5-15 kDa substances in carp seminal plasma using mass spectrometry
Summary The objective of this study was to detect substances of 5–15 kDa in carp seminal plasma using matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) and to compare mass spectrometry profiles between two common carp lines. Resolved were seminal plasma prot...
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| Veröffentlicht in: | Journal of applied ichthyology 2015-06, Vol.31 (S1), p.132-135 |
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| container_issue | S1 |
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| container_title | Journal of applied ichthyology |
| container_volume | 31 |
| creator | Westfalewicz, B. Dietrich, M. A. Irnazarow, I. Ciereszko, A. |
| description | Summary
The objective of this study was to detect substances of 5–15 kDa in carp seminal plasma using matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) and to compare mass spectrometry profiles between two common carp lines. Resolved were seminal plasma proteins using tris – tricine SDS – PAGE. Also found were 19 mass spectra peaks of carp seminal plasma. A few low‐molecular mass proteins were identified, including parvalbumin, apolipoprotein, histone H4 and H2A, and one hypothetical protein. Mass spectrometry was not useful for molecular profiling of carp breeding lines, but are potentially useful for comparative studies of cyprinids. |
| doi_str_mv | 10.1111/jai.12738 |
| format | Article |
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The objective of this study was to detect substances of 5–15 kDa in carp seminal plasma using matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) and to compare mass spectrometry profiles between two common carp lines. Resolved were seminal plasma proteins using tris – tricine SDS – PAGE. Also found were 19 mass spectra peaks of carp seminal plasma. A few low‐molecular mass proteins were identified, including parvalbumin, apolipoprotein, histone H4 and H2A, and one hypothetical protein. Mass spectrometry was not useful for molecular profiling of carp breeding lines, but are potentially useful for comparative studies of cyprinids.</description><identifier>ISSN: 0175-8659</identifier><identifier>EISSN: 1439-0426</identifier><identifier>DOI: 10.1111/jai.12738</identifier><language>eng</language><publisher>Berlin: Blackwell Publishing Ltd</publisher><subject>Cyprinus carpio ; Freshwater</subject><ispartof>Journal of applied ichthyology, 2015-06, Vol.31 (S1), p.132-135</ispartof><rights>2015 Blackwell Verlag GmbH</rights><rights>Copyright © 2015 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3188-ae0ab02cf015bb05e01618457b7c20b3791e07ebb175806c45d29207fa60c5953</citedby><cites>FETCH-LOGICAL-c3188-ae0ab02cf015bb05e01618457b7c20b3791e07ebb175806c45d29207fa60c5953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjai.12738$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjai.12738$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Westfalewicz, B.</creatorcontrib><creatorcontrib>Dietrich, M. A.</creatorcontrib><creatorcontrib>Irnazarow, I.</creatorcontrib><creatorcontrib>Ciereszko, A.</creatorcontrib><title>Identification of 5-15 kDa substances in carp seminal plasma using mass spectrometry</title><title>Journal of applied ichthyology</title><addtitle>J. Appl. Ichthyol</addtitle><description>Summary
The objective of this study was to detect substances of 5–15 kDa in carp seminal plasma using matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) and to compare mass spectrometry profiles between two common carp lines. Resolved were seminal plasma proteins using tris – tricine SDS – PAGE. Also found were 19 mass spectra peaks of carp seminal plasma. A few low‐molecular mass proteins were identified, including parvalbumin, apolipoprotein, histone H4 and H2A, and one hypothetical protein. Mass spectrometry was not useful for molecular profiling of carp breeding lines, but are potentially useful for comparative studies of cyprinids.</description><subject>Cyprinus carpio</subject><subject>Freshwater</subject><issn>0175-8659</issn><issn>1439-0426</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNp10LlOxDAQBmALgcRyFLyBJRoosjtO4iMl4lgWISi4SssxDvKSC08i2LfhWXgysixQIOFmmu8fjX9C9hiM2fAmc-PHLJaJWiMjliZZBGks1skImOSREjzbJFuIcwBQMhMjcjd7dHXnC29N55uaNgXlEeMf788nhmKfY2dq65D6mloTWoqu8rUpaVsarAzt0ddPtDKIFFtnu9BUrguLHbJRmBLd7vfcJndnp7fH59Hl9XR2fHQZ2YQpFRkHJofYFsB4ngN3wARTKZe5tDHkicyYA-nyfDhegbApf4yzGGRhBFie8WSbHKz2tqF56R12uvJoXVma2jU9aiaBpZmSakn3_9B504fhK4MSQxVxzMVSHa6UDQ1icIVug69MWGgGelmwHgrWXwUPdrKyr750i_-hvjia_SSiVcJj595-EyY8ayETyfXD1VSfq-wG7q8edJp8AlWTipU</recordid><startdate>201506</startdate><enddate>201506</enddate><creator>Westfalewicz, B.</creator><creator>Dietrich, M. A.</creator><creator>Irnazarow, I.</creator><creator>Ciereszko, A.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7SN</scope><scope>7TN</scope><scope>7U7</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>201506</creationdate><title>Identification of 5-15 kDa substances in carp seminal plasma using mass spectrometry</title><author>Westfalewicz, B. ; Dietrich, M. A. ; Irnazarow, I. ; Ciereszko, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3188-ae0ab02cf015bb05e01618457b7c20b3791e07ebb175806c45d29207fa60c5953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Cyprinus carpio</topic><topic>Freshwater</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Westfalewicz, B.</creatorcontrib><creatorcontrib>Dietrich, M. A.</creatorcontrib><creatorcontrib>Irnazarow, I.</creatorcontrib><creatorcontrib>Ciereszko, A.</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Ecology Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Journal of applied ichthyology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Westfalewicz, B.</au><au>Dietrich, M. A.</au><au>Irnazarow, I.</au><au>Ciereszko, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of 5-15 kDa substances in carp seminal plasma using mass spectrometry</atitle><jtitle>Journal of applied ichthyology</jtitle><addtitle>J. Appl. Ichthyol</addtitle><date>2015-06</date><risdate>2015</risdate><volume>31</volume><issue>S1</issue><spage>132</spage><epage>135</epage><pages>132-135</pages><issn>0175-8659</issn><eissn>1439-0426</eissn><abstract>Summary
The objective of this study was to detect substances of 5–15 kDa in carp seminal plasma using matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) and to compare mass spectrometry profiles between two common carp lines. Resolved were seminal plasma proteins using tris – tricine SDS – PAGE. Also found were 19 mass spectra peaks of carp seminal plasma. A few low‐molecular mass proteins were identified, including parvalbumin, apolipoprotein, histone H4 and H2A, and one hypothetical protein. Mass spectrometry was not useful for molecular profiling of carp breeding lines, but are potentially useful for comparative studies of cyprinids.</abstract><cop>Berlin</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/jai.12738</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | Access via Wiley Online Library |
| subjects | Cyprinus carpio Freshwater |
| title | Identification of 5-15 kDa substances in carp seminal plasma using mass spectrometry |
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