Identification of 5-15 kDa substances in carp seminal plasma using mass spectrometry

Summary The objective of this study was to detect substances of 5–15 kDa in carp seminal plasma using matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) and to compare mass spectrometry profiles between two common carp lines. Resolved were seminal plasma proteins using tris – tricine SDS – PAGE. Also found were 19 mass spectra peaks of carp seminal plasma. A few low‐molecular mass proteins were identified, including parvalbumin, apolipoprotein, histone H4 and H2A, and one hypothetical protein. Mass spectrometry was not useful for molecular profiling of carp breeding lines, but are potentially useful for comparative studies of cyprinids.