Antimicrobial activities and action mechanism studies of transportan 10 and its analogues against multidrug-resistant bacteria

The increased emergence of multidrug‐resistant bacteria is perceived as a critical public health threat, creating an urgent need for the development of novel classes of antimicrobials. Cell‐penetrating peptides that share common features with antimicrobial peptides have been found to have antimicrobial activity and are currently being considered as potential alternatives to antibiotics. Transportan 10 is a chimeric cell‐penetrating peptide that has been reported to transport biologically relevant cargoes into mammalian cells and cause damage to microbial membranes. In this study, we designed a series of TP10 analogues and studied their structure‐activity relationships. We first evaluated the antimicrobial activities of these compounds against multidrug‐resistant bacteria, which are responsible for most nosocomial infections. Our results showed that several of these compounds had potent antimicrobial and biofilm‐inhibiting activities. We also measured the toxicity of these compounds, finding that Lys substitution could increase the antimicrobial activity but significantly enhanced the cytotoxicity. Pro introduction could reduce the cytotoxicity but disrupted the helical structure, resulting in a loss of activity. In the mechanistic studies, TP10 killed bacteria by membrane‐active and DNA‐binding activities. In conclusion, TP10 and its analogues could be developed into promising antibiotic candidates for the treatment of infections caused by multidrug‐resistant bacteria. Copyright © 2015 European Peptide Society and John Wiley & Sons, Ltd. Transportan 10 (TP10) and some of its analogues exhibited potent antimicrobial activities against multidrug‐resistant bacteria. The scanning electron microscopy showed the effect of TP10 on the morphology of the Escherichia coli bacterial membrane. It could kill the bacteria by disrupting the membrane and was not affected by the common resistance mechanisms found in bacteria. TP10 and its analogues could be developed into promising antibiotic candidates for the treatment of infections caused by multidrug‐resistant bacteria.