Ratiometric Activatable Cell-Penetrating Peptides Provide Rapid InVivo Readout of Thrombin Activation
In real time: thrombin activation in vivo can be imaged in real time with ratiometric activatable cell penetrating peptides (RACPPs). RACPPs are designed to combine 1) dual-emission ratioing, 2) far red to infrared wavelengths for in vivo mammalian imaging, and 3) cleavage-dependent spatial localiza...
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| Veröffentlicht in: | Angewandte Chemie International Edition 2013-01, Vol.52 (1), p.325-330 |
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| container_issue | 1 |
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| container_title | Angewandte Chemie International Edition |
| container_volume | 52 |
| creator | Whitney, Michael Savariar, Elamprakash N Friedman, Beth Levin, Rachel A Crisp, Jessica L Glasgow, Heather L Lefkowitz, Roy Adams, Stephen R Steinbach, Paul Nashi, Nadia Nguyen, Quyen T Tsien, Roger Y |
| description | In real time: thrombin activation in vivo can be imaged in real time with ratiometric activatable cell penetrating peptides (RACPPs). RACPPs are designed to combine 1) dual-emission ratioing, 2) far red to infrared wavelengths for in vivo mammalian imaging, and 3) cleavage-dependent spatial localization. The most advanced RACPP uses norleucine (Nle)-TPRSFL as a linker that increases sensitivity to thrombin by about 90-fold. |
| doi_str_mv | 10.1002/anie.201205721 |
| format | Article |
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| ispartof | Angewandte Chemie International Edition, 2013-01, Vol.52 (1), p.325-330 |
| issn | 1433-7851 1521-3773 |
| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Activation Infrared Localization Norleucine Peptides Real time Thrombin Wavelengths |
| title | Ratiometric Activatable Cell-Penetrating Peptides Provide Rapid InVivo Readout of Thrombin Activation |
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