Ratiometric Activatable Cell-Penetrating Peptides Provide Rapid InVivo Readout of Thrombin Activation

Ratiometric Activatable Cell-Penetrating Peptides Provide Rapid InVivo Readout of Thrombin Activation

In real time: thrombin activation in vivo can be imaged in real time with ratiometric activatable cell penetrating peptides (RACPPs). RACPPs are designed to combine 1) dual-emission ratioing, 2) far red to infrared wavelengths for in vivo mammalian imaging, and 3) cleavage-dependent spatial localiza...

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Veröffentlicht in:Angewandte Chemie International Edition 2013-01, Vol.52 (1), p.325-330
Hauptverfasser: Whitney, Michael, Savariar, Elamprakash N, Friedman, Beth, Levin, Rachel A, Crisp, Jessica L, Glasgow, Heather L, Lefkowitz, Roy, Adams, Stephen R, Steinbach, Paul, Nashi, Nadia, Nguyen, Quyen T, Tsien, Roger Y
Format: Artikel
Sprache:eng
Schlagworte:
Activation
Infrared
Localization
Norleucine
Peptides
Real time
Thrombin
Wavelengths
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Beschreibung
Zusammenfassung:In real time: thrombin activation in vivo can be imaged in real time with ratiometric activatable cell penetrating peptides (RACPPs). RACPPs are designed to combine 1) dual-emission ratioing, 2) far red to infrared wavelengths for in vivo mammalian imaging, and 3) cleavage-dependent spatial localization. The most advanced RACPP uses norleucine (Nle)-TPRSFL as a linker that increases sensitivity to thrombin by about 90-fold.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201205721