Sequencing the Sixth Base (5-Hydroxymethylcytosine): Selective DNA Oxidation Enables Base-Pair Resolution

Sequencing the Sixth Base (5-Hydroxymethylcytosine): Selective DNA Oxidation Enables Base-Pair Resolution

Sodium bisulfite promotes both the deformylative deamination of 5‐formylcytosine (5fC) and the decarboxylative deamination of 5‐carboxylcytosine (5caC; see picture). By coupling this bisulfite chemistry with selective oxidations of individual DNA bases, new methods allow, for the first time, the seq...

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Veröffentlicht in:Angewandte Chemie International Edition 2012-10, Vol.51 (43), p.10704-10707
Hauptverfasser: Schüler, Peter, Miller, Aubry K.
Format: Artikel
Sprache:eng
Schlagworte:
5-hydroxymethylcytosine
5-Methylcytosine - analogs & derivatives
Base Pairing
Cytosine - analogs & derivatives
Cytosine - chemistry
Deamination
Deformation
Deoxyribonucleic acid
DNA
DNA - chemistry
enzymes
Gene sequencing
Joining
Oxidation
Oxidation-Reduction
Pictures
Sequence Analysis, DNA
sequencing
Sodium
Sulfites - chemistry
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Beschreibung
Zusammenfassung:Sodium bisulfite promotes both the deformylative deamination of 5‐formylcytosine (5fC) and the decarboxylative deamination of 5‐carboxylcytosine (5caC; see picture). By coupling this bisulfite chemistry with selective oxidations of individual DNA bases, new methods allow, for the first time, the sequencing of 5‐hydroxymethylcytosine (5hmC) with single‐base‐pair resolution.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201204768