Triple-Color Super-Resolution Imaging of Live Cells: Resolving Submicroscopic Receptor Organization in the Plasma Membrane

In living color: Efficient intracellular covalent labeling of proteins with a photoswitchable dye using the HaloTag for dSTORM super‐resolution imaging in live cells is described. The dynamics of cellular nanostructures at the plasma membrane were monitored with a time resolution of a few seconds. I...

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Veröffentlicht in:	Angewandte Chemie International Edition 2012-05, Vol.51 (20), p.4868-4871
Hauptverfasser:	Wilmes, Stephan, Staufenbiel, Markus, Liße, Domenik, Richter, Christian P., Beutel, Oliver, Busch, Karin B., Hess, Samuel T., Piehler, Jacob
Format:	Artikel
Sprache:	eng
Schlagworte:	Actins - chemistry Actins - metabolism Cell Membrane - chemistry Cell Membrane - metabolism Color Covalence Cytoskeleton - chemistry Cytoskeleton - metabolism Dyes Dynamics fluorescence Fluorescent Dyes - chemistry Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - metabolism HeLa Cells Humans Imaging imaging agents membrane proteins Membranes Microscopy, Fluorescence - methods Nanostructure Nanostructures - chemistry Organizations protein-protein interactions Receptors single-molecule studies Transfection
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Zusammenfassung:	In living color: Efficient intracellular covalent labeling of proteins with a photoswitchable dye using the HaloTag for dSTORM super‐resolution imaging in live cells is described. The dynamics of cellular nanostructures at the plasma membrane were monitored with a time resolution of a few seconds. In combination with dual‐color FPALM imaging, submicroscopic receptor organization within the context of the membrane skeleton was resolved (see figure).
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201200853