Identification of a Selective, Activity-Based Probe for Glyceraldehyde 3-Phosphate Dehydrogenases

Exceptionally specific: The natural‐product‐like structural complexity of a bicyclic hydantoin was exploited to generate the novel, highly specific activity‐based profiling probe (ABPP) Mrl‐Rh (Rh=rhodamine; see picture) for glyceraldehyde 3‐phosphate dehydrogenases. This probe can be used to invest...

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Veröffentlicht in:	Angewandte Chemie International Edition 2012-05, Vol.51 (21), p.5230-5233
Hauptverfasser:	Kaschani, Farnusch, Clerc, Jérôme, Krahn, Daniel, Bier, David, Hong, Tram Ngoc, Ottmann, Christian, Niessen, Sherry, Colby, Tom, van der Hoorn, Renier A. L., Kaiser, Markus
Format:	Artikel
Sprache:	eng
Schlagworte:	activity-based protein profiling chemical biology chemical proteomics Chromatography, Liquid Complexity Electrophoresis, Polyacrylamide Gel Enzymes Feasibility Studies GAPDH Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism Hydantoin inhibitors Molecular Probes Pictures Profiling Tandem Mass Spectrometry
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Beschreibung
Zusammenfassung:	Exceptionally specific: The natural‐product‐like structural complexity of a bicyclic hydantoin was exploited to generate the novel, highly specific activity‐based profiling probe (ABPP) Mrl‐Rh (Rh=rhodamine; see picture) for glyceraldehyde 3‐phosphate dehydrogenases. This probe can be used to investigate activity changes of this enzyme class during plant–pathogen interactions.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201107276