Synthesis of Autophagosomal Marker Protein LC3-II under Detergent-Free Conditions

Synthesis of Autophagosomal Marker Protein LC3-II under Detergent-Free Conditions

Just add oil: A new detergent‐free method was developed to synthesize lipidated proteins using a light‐activatable solubilizing side chain (in dashed circle, see scheme) to assist the ligation of the lipopeptides. This method allows the efficient preparation of a phosphatidylethanolamine‐conjugated...

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Veröffentlicht in:Angewandte Chemie International Edition 2013-04, Vol.52 (18), p.4858-4862
Hauptverfasser: Huang, Yi-Chao, Li, Yi-Ming, Chen, Yang, Pan, Man, Li, Yi-Tong, Yu, Li, Guo, Qing-Xiang, Liu, Lei
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Autophagy
Chains
Circular Dichroism
Control
Derivatives
Detergents - chemistry
Humans
Hydrazines - chemistry
LC3-II
lipoproteins
Lysosomes - metabolism
Markers
Microtubule-Associated Proteins - chemical synthesis
Microtubule-Associated Proteins - chemistry
Microtubule-Associated Proteins - genetics
Microtubule-Associated Proteins - metabolism
Molecular Sequence Data
Peptides - chemical synthesis
Peptides - chemistry
Phosphatidylethanolamines - chemistry
protein expression
protein ligation
Proteins
Synthesis
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Beschreibung
Zusammenfassung:Just add oil: A new detergent‐free method was developed to synthesize lipidated proteins using a light‐activatable solubilizing side chain (in dashed circle, see scheme) to assist the ligation of the lipopeptides. This method allows the efficient preparation of a phosphatidylethanolamine‐conjugated autophagosomal marker protein (LC3‐II) as well as labeled derivatives of LC3‐II, which can be used to study autophagy regulation.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201209523