Suppression of Gene Expression by G-Quadruplexes in Open Reading Frames Depends on G-Quadruplex Stability

Suppressing the urge to translate: Sequences with the potential to form G‐quadruplexes were identified in the open reading frames of E. coli genes. These sequences were found to form parallel G‐quadruplexes and to suppress translation (see picture) of the mRNAs into proteins both in vitro and in cel...

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Veröffentlicht in:	Angewandte Chemie International Edition 2013-05, Vol.52 (21), p.5522-5526
Hauptverfasser:	Endoh, Tamaki, Kawasaki, Yu, Sugimoto, Naoki
Format:	Artikel
Sprache:	eng
Schlagworte:	5' Untranslated Regions - genetics Cell Culture Techniques Electrophoresis, Polyacrylamide Gel Escherichia coli - genetics Frames G-Quadruplexes Gene Expression Gene sequencing Genes Genes, Reporter Humans Luciferases, Firefly - genetics Luciferases, Renilla - genetics MCF-7 Cells open reading frames Open Reading Frames - genetics Optical Imaging Peptide Chain Elongation, Translational - genetics Plasmids protein translation Proteins Retarding Ribosomes - genetics RNA recognition Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet Stability thermodynamic stability Thermodynamics Translations
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Zusammenfassung:	Suppressing the urge to translate: Sequences with the potential to form G‐quadruplexes were identified in the open reading frames of E. coli genes. These sequences were found to form parallel G‐quadruplexes and to suppress translation (see picture) of the mRNAs into proteins both in vitro and in cells.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201300058