Dioscorea bulbifera Mediated Synthesis of Novel Aucore Agshell Nanoparticles with Potent Antibiofilm and Antileishmanial Activity

Dioscorea bulbifera is a potent medicinal plant used in both Indian and Chinese traditional medicine owing to its rich phytochemical diversity. Herein, we report the rapid synthesis of novel sub(Aucore) sub(Agshell) nanoparticles by D. bulbifera tuber extract (DBTE). sub(Aucore) sub(Agshell) NPs synthesis was completed within 5 h showing a prominent peak at 540 nm. HRTEM analysis revealed 9 nm inner core of elemental gold covered by a silver shell giving a total particle diameter upto 15 nm. sub(Aucore) sub(Agshell) NPs were comprised of 57.34 plus or minus 1.01 % gold and 42.66 plus or minus 0.97 % silver of the total mass. sub(Aucore) sub(Agshell) NPs showed highest biofilm inhibition upto 83.68 plus or minus 0.09 % against A. baumannii . Biofilms of P. aeruginosa , E. coli , and S. aureus were inhibited up to 18.93 plus or minus 1.94 %, 22.33 plus or minus 0.56 %, and 30.70 plus or minus 1.33 %, respectively. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) confirmed unregulated cellular efflux through pore formation leading to cell death. Potent antileishmanial activity of sub(Aucore) sub(Agshell) NPs ( MIC=32 mu g/mL) was confirmed by MTT assay. Further SEM micrographs showed pronounced deformity in the spindle shaped cellular morphology changing to spherical. This is the first report of synthesis, characterization, antibiofilm, and antileishmanial activity of sub(Aucore) sub(Agshell) NPs synthesized by D. bulbifera .