Development of a MALDI-TOF MS Strategy for the High-Throughput Analysis of Biomarkers: On-Target Aptamer Immobilization and Laser-Accelerated Proteolysis

Development of a MALDI-TOF MS Strategy for the High-Throughput Analysis of Biomarkers: On-Target Aptamer Immobilization and Laser-Accelerated Proteolysis

An aptamer‐based strategy was developed for the high‐throughput analysis of protein biomarkers, such as lysozyme, by on‐target MALDI‐TOF MS. The aptamers were immobilized on the target plate through formation of covalent bonds with a stable and porous gold layer. An infrared laser was subsequently a...

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Veröffentlicht in:Angewandte Chemie International Edition 2013-06, Vol.52 (23), p.6055-6058
Hauptverfasser: Zhang, Xueyang, Zhu, Shaochun, Xiong, Ya, Deng, Chunhui, Zhang, Xiangmin
Format: Artikel
Sprache:eng
Schlagworte:
aptamers
Aptamers, Peptide
Biomarkers
Covalent bonds
gold
Gold - chemistry
High-Throughput Screening Assays
Human
Humans
Immobilization
lysozyme
mass spectrometry
nanoparticles
Proteins
Proteolysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Strategy
Urine
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Zusammenfassung:An aptamer‐based strategy was developed for the high‐throughput analysis of protein biomarkers, such as lysozyme, by on‐target MALDI‐TOF MS. The aptamers were immobilized on the target plate through formation of covalent bonds with a stable and porous gold layer. An infrared laser was subsequently applied for fast proteolysis (see picture). High sensitivities were observed both in standard solutions and human urine.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201300566