Analysis of Microarrays by MALDI-TOF MS

Ligand libraries can be printed onto a sandwich composed of activated lipids embedded in a hydrophobic layer conjugated to an indium–tin oxide (ITO) surface. Arrays produced this way can be analyzed by fluorescence spectroscopy and mass spectrometry. Applications include the assignment of enzyme spe...

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Veröffentlicht in:	Angewandte Chemie International Edition 2013-07, Vol.52 (29), p.7477-7481
Hauptverfasser:	Beloqui, Ana, Calvo, Javier, Serna, Sonia, Yan, Shi, Wilson, Iain B. H., Martin-Lomas, Manuel, Reichardt, Niels Christian
Format:	Artikel
Sprache:	eng
Schlagworte:	Arrays Fluorescence Indium-Tin lectins Lectins - analysis Ligands Lipids Lipids - chemistry mass spectrometry microarrays Oxides Profiling Protein Array Analysis Proteomics Spectrometry, Fluorescence Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Surface chemistry Surface Properties Tin Compounds - chemistry transferases Transferases - metabolism
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Beschreibung
Zusammenfassung:	Ligand libraries can be printed onto a sandwich composed of activated lipids embedded in a hydrophobic layer conjugated to an indium–tin oxide (ITO) surface. Arrays produced this way can be analyzed by fluorescence spectroscopy and mass spectrometry. Applications include the assignment of enzyme specificity, the profiling of glycoforms and the identification of lectins.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201302455