Vinylsulfonamide and Acrylamide Modification of DNA for Cross-linking with Proteins
Bioorthogonal covalent cross‐linking of DNA‐binding proteins (p53) to DNA was achieved through novel DNA probes bearing a reactive vinylsulfonamide (VS) group. The VS‐modified dCTP served as building block for polymerase synthesis of modified DNA, which was readily conjugated with cysteine‐containin...
Gespeichert in:
| Veröffentlicht in: | Angewandte Chemie International Edition 2013-09, Vol.52 (40), p.10515-10518 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 10518 |
|---|---|
| container_issue | 40 |
| container_start_page | 10515 |
| container_title | Angewandte Chemie International Edition |
| container_volume | 52 |
| creator | Dadová, Jitka Orság, Petr Pohl, Radek Brázdová, Marie Fojta, Miroslav Hocek, Michal |
| description | Bioorthogonal covalent cross‐linking of DNA‐binding proteins (p53) to DNA was achieved through novel DNA probes bearing a reactive vinylsulfonamide (VS) group. The VS‐modified dCTP served as building block for polymerase synthesis of modified DNA, which was readily conjugated with cysteine‐containing peptides and proteins by Michael addition. |
| doi_str_mv | 10.1002/anie.201303577 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1701048082</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3083362271</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5147-e46415815b2a86b6d091f513404f3a96408ab18caa52ca8ed008cda8c0c794393</originalsourceid><addsrcrecordid>eNqN0U1v1DAQBmALgWgpXDmiSFy4ZJmJ7dg-rpZ-Se1SqaXlZnkdB9xm7WInKvvvySplhbi0J9vSM680fgl5jzBDgOqzCd7NKkAKlAvxguwjr7CkQtCX451RWgrJcY-8yfl29FJC_ZrsVVRRpSjdJ5fXPmy6PHRtDGbtG1eY0BRzmzbd9DyPjW-9Nb2PoYht8WU5L9qYikWKOZedD3c-_CgefP-zuEixdz7kt-RVa7rs3j2eB-Tb0eHV4qQ8-3p8upiflZYjE6VjNUMuka8qI-tV3YDCliNlwFpqVM1AmhVKawyvrJGuAZC2MdKCFYqNGxyQT1PufYq_Bpd7vfbZuq4zwcUhaxSAwCTI6mnKmKyQC6TPoFTyWingI_34H72NQwrjzlslUNa1hFHNJmW3X5Zcq--TX5u00Qh6W6Lelqh3JY4DHx5jh9XaNTv-t7URqAk8-M5tnojT8-Xp4b_h5TTrc-9-72ZNutO1oILrm-WxxpOj75fnSuor-gctaLVI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1437186680</pqid></control><display><type>article</type><title>Vinylsulfonamide and Acrylamide Modification of DNA for Cross-linking with Proteins</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Dadová, Jitka ; Orság, Petr ; Pohl, Radek ; Brázdová, Marie ; Fojta, Miroslav ; Hocek, Michal</creator><creatorcontrib>Dadová, Jitka ; Orság, Petr ; Pohl, Radek ; Brázdová, Marie ; Fojta, Miroslav ; Hocek, Michal</creatorcontrib><description>Bioorthogonal covalent cross‐linking of DNA‐binding proteins (p53) to DNA was achieved through novel DNA probes bearing a reactive vinylsulfonamide (VS) group. The VS‐modified dCTP served as building block for polymerase synthesis of modified DNA, which was readily conjugated with cysteine‐containing peptides and proteins by Michael addition.</description><edition>International ed. in English</edition><identifier>ISSN: 1433-7851</identifier><identifier>EISSN: 1521-3773</identifier><identifier>DOI: 10.1002/anie.201303577</identifier><identifier>PMID: 23939933</identifier><identifier>CODEN: ACIEAY</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Acrylamide ; Acrylamide - chemical synthesis ; Acrylamide - chemistry ; Bearing ; bioorthogonal chemistry ; Covalence ; Cross-Linking Reagents - chemistry ; Crosslinking ; Deoxyribonucleic acid ; DNA ; DNA - chemical synthesis ; DNA - chemistry ; DNA polymerase ; DNA-Directed DNA Polymerase - chemistry ; Ethylenes - chemistry ; Michael additions ; Models, Molecular ; Polymerase ; Proteins ; Proteins - chemistry ; Proteins - metabolism ; Sulfonamides - chemical synthesis ; Sulfonamides - chemistry ; Sulfonic Acids - chemistry ; Synthesis ; Vinyl Compounds - chemical synthesis ; Vinyl Compounds - chemistry</subject><ispartof>Angewandte Chemie International Edition, 2013-09, Vol.52 (40), p.10515-10518</ispartof><rights>Copyright © 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5147-e46415815b2a86b6d091f513404f3a96408ab18caa52ca8ed008cda8c0c794393</citedby><cites>FETCH-LOGICAL-c5147-e46415815b2a86b6d091f513404f3a96408ab18caa52ca8ed008cda8c0c794393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fanie.201303577$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fanie.201303577$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23939933$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dadová, Jitka</creatorcontrib><creatorcontrib>Orság, Petr</creatorcontrib><creatorcontrib>Pohl, Radek</creatorcontrib><creatorcontrib>Brázdová, Marie</creatorcontrib><creatorcontrib>Fojta, Miroslav</creatorcontrib><creatorcontrib>Hocek, Michal</creatorcontrib><title>Vinylsulfonamide and Acrylamide Modification of DNA for Cross-linking with Proteins</title><title>Angewandte Chemie International Edition</title><addtitle>Angew. Chem. Int. Ed</addtitle><description>Bioorthogonal covalent cross‐linking of DNA‐binding proteins (p53) to DNA was achieved through novel DNA probes bearing a reactive vinylsulfonamide (VS) group. The VS‐modified dCTP served as building block for polymerase synthesis of modified DNA, which was readily conjugated with cysteine‐containing peptides and proteins by Michael addition.</description><subject>Acrylamide</subject><subject>Acrylamide - chemical synthesis</subject><subject>Acrylamide - chemistry</subject><subject>Bearing</subject><subject>bioorthogonal chemistry</subject><subject>Covalence</subject><subject>Cross-Linking Reagents - chemistry</subject><subject>Crosslinking</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - chemical synthesis</subject><subject>DNA - chemistry</subject><subject>DNA polymerase</subject><subject>DNA-Directed DNA Polymerase - chemistry</subject><subject>Ethylenes - chemistry</subject><subject>Michael additions</subject><subject>Models, Molecular</subject><subject>Polymerase</subject><subject>Proteins</subject><subject>Proteins - chemistry</subject><subject>Proteins - metabolism</subject><subject>Sulfonamides - chemical synthesis</subject><subject>Sulfonamides - chemistry</subject><subject>Sulfonic Acids - chemistry</subject><subject>Synthesis</subject><subject>Vinyl Compounds - chemical synthesis</subject><subject>Vinyl Compounds - chemistry</subject><issn>1433-7851</issn><issn>1521-3773</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0U1v1DAQBmALgWgpXDmiSFy4ZJmJ7dg-rpZ-Se1SqaXlZnkdB9xm7WInKvvvySplhbi0J9vSM680fgl5jzBDgOqzCd7NKkAKlAvxguwjr7CkQtCX451RWgrJcY-8yfl29FJC_ZrsVVRRpSjdJ5fXPmy6PHRtDGbtG1eY0BRzmzbd9DyPjW-9Nb2PoYht8WU5L9qYikWKOZedD3c-_CgefP-zuEixdz7kt-RVa7rs3j2eB-Tb0eHV4qQ8-3p8upiflZYjE6VjNUMuka8qI-tV3YDCliNlwFpqVM1AmhVKawyvrJGuAZC2MdKCFYqNGxyQT1PufYq_Bpd7vfbZuq4zwcUhaxSAwCTI6mnKmKyQC6TPoFTyWingI_34H72NQwrjzlslUNa1hFHNJmW3X5Zcq--TX5u00Qh6W6Lelqh3JY4DHx5jh9XaNTv-t7URqAk8-M5tnojT8-Xp4b_h5TTrc-9-72ZNutO1oILrm-WxxpOj75fnSuor-gctaLVI</recordid><startdate>20130927</startdate><enddate>20130927</enddate><creator>Dadová, Jitka</creator><creator>Orság, Petr</creator><creator>Pohl, Radek</creator><creator>Brázdová, Marie</creator><creator>Fojta, Miroslav</creator><creator>Hocek, Michal</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>K9.</scope><scope>7X8</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>20130927</creationdate><title>Vinylsulfonamide and Acrylamide Modification of DNA for Cross-linking with Proteins</title><author>Dadová, Jitka ; Orság, Petr ; Pohl, Radek ; Brázdová, Marie ; Fojta, Miroslav ; Hocek, Michal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5147-e46415815b2a86b6d091f513404f3a96408ab18caa52ca8ed008cda8c0c794393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acrylamide</topic><topic>Acrylamide - chemical synthesis</topic><topic>Acrylamide - chemistry</topic><topic>Bearing</topic><topic>bioorthogonal chemistry</topic><topic>Covalence</topic><topic>Cross-Linking Reagents - chemistry</topic><topic>Crosslinking</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - chemical synthesis</topic><topic>DNA - chemistry</topic><topic>DNA polymerase</topic><topic>DNA-Directed DNA Polymerase - chemistry</topic><topic>Ethylenes - chemistry</topic><topic>Michael additions</topic><topic>Models, Molecular</topic><topic>Polymerase</topic><topic>Proteins</topic><topic>Proteins - chemistry</topic><topic>Proteins - metabolism</topic><topic>Sulfonamides - chemical synthesis</topic><topic>Sulfonamides - chemistry</topic><topic>Sulfonic Acids - chemistry</topic><topic>Synthesis</topic><topic>Vinyl Compounds - chemical synthesis</topic><topic>Vinyl Compounds - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dadová, Jitka</creatorcontrib><creatorcontrib>Orság, Petr</creatorcontrib><creatorcontrib>Pohl, Radek</creatorcontrib><creatorcontrib>Brázdová, Marie</creatorcontrib><creatorcontrib>Fojta, Miroslav</creatorcontrib><creatorcontrib>Hocek, Michal</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Angewandte Chemie International Edition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dadová, Jitka</au><au>Orság, Petr</au><au>Pohl, Radek</au><au>Brázdová, Marie</au><au>Fojta, Miroslav</au><au>Hocek, Michal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vinylsulfonamide and Acrylamide Modification of DNA for Cross-linking with Proteins</atitle><jtitle>Angewandte Chemie International Edition</jtitle><addtitle>Angew. Chem. Int. Ed</addtitle><date>2013-09-27</date><risdate>2013</risdate><volume>52</volume><issue>40</issue><spage>10515</spage><epage>10518</epage><pages>10515-10518</pages><issn>1433-7851</issn><eissn>1521-3773</eissn><coden>ACIEAY</coden><abstract>Bioorthogonal covalent cross‐linking of DNA‐binding proteins (p53) to DNA was achieved through novel DNA probes bearing a reactive vinylsulfonamide (VS) group. The VS‐modified dCTP served as building block for polymerase synthesis of modified DNA, which was readily conjugated with cysteine‐containing peptides and proteins by Michael addition.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>23939933</pmid><doi>10.1002/anie.201303577</doi><tpages>4</tpages><edition>International ed. in English</edition></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1433-7851 |
| ispartof | Angewandte Chemie International Edition, 2013-09, Vol.52 (40), p.10515-10518 |
| issn | 1433-7851 1521-3773 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1701048082 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Acrylamide Acrylamide - chemical synthesis Acrylamide - chemistry Bearing bioorthogonal chemistry Covalence Cross-Linking Reagents - chemistry Crosslinking Deoxyribonucleic acid DNA DNA - chemical synthesis DNA - chemistry DNA polymerase DNA-Directed DNA Polymerase - chemistry Ethylenes - chemistry Michael additions Models, Molecular Polymerase Proteins Proteins - chemistry Proteins - metabolism Sulfonamides - chemical synthesis Sulfonamides - chemistry Sulfonic Acids - chemistry Synthesis Vinyl Compounds - chemical synthesis Vinyl Compounds - chemistry |
| title | Vinylsulfonamide and Acrylamide Modification of DNA for Cross-linking with Proteins |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T21%3A40%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Vinylsulfonamide%20and%20Acrylamide%20Modification%20of%20DNA%20for%20Cross-linking%20with%20Proteins&rft.jtitle=Angewandte%20Chemie%20International%20Edition&rft.au=Dadov%C3%A1,%20Jitka&rft.date=2013-09-27&rft.volume=52&rft.issue=40&rft.spage=10515&rft.epage=10518&rft.pages=10515-10518&rft.issn=1433-7851&rft.eissn=1521-3773&rft.coden=ACIEAY&rft_id=info:doi/10.1002/anie.201303577&rft_dat=%3Cproquest_cross%3E3083362271%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1437186680&rft_id=info:pmid/23939933&rfr_iscdi=true |