Vinylsulfonamide and Acrylamide Modification of DNA for Cross-linking with Proteins

Bioorthogonal covalent cross‐linking of DNA‐binding proteins (p53) to DNA was achieved through novel DNA probes bearing a reactive vinylsulfonamide (VS) group. The VS‐modified dCTP served as building block for polymerase synthesis of modified DNA, which was readily conjugated with cysteine‐containin...

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Veröffentlicht in:	Angewandte Chemie International Edition 2013-09, Vol.52 (40), p.10515-10518
Hauptverfasser:	Dadová, Jitka, Orság, Petr, Pohl, Radek, Brázdová, Marie, Fojta, Miroslav, Hocek, Michal
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrylamide Acrylamide - chemical synthesis Acrylamide - chemistry Bearing bioorthogonal chemistry Covalence Cross-Linking Reagents - chemistry Crosslinking Deoxyribonucleic acid DNA DNA - chemical synthesis DNA - chemistry DNA polymerase DNA-Directed DNA Polymerase - chemistry Ethylenes - chemistry Michael additions Models, Molecular Polymerase Proteins Proteins - chemistry Proteins - metabolism Sulfonamides - chemical synthesis Sulfonamides - chemistry Sulfonic Acids - chemistry Synthesis Vinyl Compounds - chemical synthesis Vinyl Compounds - chemistry
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Zusammenfassung:	Bioorthogonal covalent cross‐linking of DNA‐binding proteins (p53) to DNA was achieved through novel DNA probes bearing a reactive vinylsulfonamide (VS) group. The VS‐modified dCTP served as building block for polymerase synthesis of modified DNA, which was readily conjugated with cysteine‐containing peptides and proteins by Michael addition.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201303577