Real-Time Monitoring of Auxin Vesicular Exocytotic Efflux from Single Plant Protoplasts by Amperometry at Microelectrodes Decorated with Nanowires

Recent biochemical results suggest that auxin (IAA) efflux is mediated by a vesicular cycling mechanism, but no direct detection of vesicular IAA release from single plant cells in real‐time has been possible up to now. A TiC@C/Pt‐QANFA micro‐electrochemical sensor has been developed with high sensitivity in detection of IAA, and it allows real‐time monitoring and quantification of the quantal release of auxin from single plant protoplast by exocytosis. Auxin(IAA) efflux has been shown by recent biochemical results to be mediated by a vesicular cycling mechanism, but up to now there was no means for direct detection of IAA release from single plant cells in real‐time. A novel TiC@C/Pt‐QANFA micro‐electrochemical sensor has high sensitivity in detection of IAA, and allows real‐time monitoring and quantification of the quantal release of auxin from single plant protoplast by exocytosis.