A Facile Strategy for Selective Incorporation of Phosphoserine into Histones

A Facile Strategy for Selective Incorporation of Phosphoserine into Histones

Phosphoserine incorporation: A general strategy for producing recombinant histones with site‐specific serine phosphorylation is developed by engineering phosphoseryl‐tRNA synthetase (SepRS) and elongation factor Tu (EF‐Tu; see picture). Serine‐phosphorylated nucleosomes provide direct evidence for c...

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Veröffentlicht in:Angewandte Chemie International Edition 2013-05, Vol.52 (22), p.5771-5775
Hauptverfasser: Lee, Sangsik, Oh, Seunghee, Yang, Aerin, Kim, Jihyo, Söll, Dieter, Lee, Daeyoup, Park, Hee-Sung
Format: Artikel
Sprache:eng
Schlagworte:
Acetylation
Crosstalk
Elongation
Histones
Histones - chemistry
Histones - metabolism
Phosphorylation
phosphoserine
Phosphoserine - chemistry
Phosphoserine - metabolism
Pictures
protein engineering
Protein Processing, Post-Translational
Recombinant
Strategy
unnatural amino acids
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Zusammenfassung:Phosphoserine incorporation: A general strategy for producing recombinant histones with site‐specific serine phosphorylation is developed by engineering phosphoseryl‐tRNA synthetase (SepRS) and elongation factor Tu (EF‐Tu; see picture). Serine‐phosphorylated nucleosomes provide direct evidence for crosstalk between phosphorylation and acetylation in histones.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201300531