NeuO: a Fluorescent Chemical Probe for Live Neuron Labeling

To address existing limitations in live neuron imaging, we have developed NeuO, a novel cell‐permeable fluorescent probe with an unprecedented ability to label and image live neurons selectively over other cells in the brain. NeuO enables robust live neuron imaging and isolation in vivo and in vitro across species; its versatility and ease of use sets the basis for its development in a myriad of neuronal targeting applications. Selective labeling of live neurons over other brain cells was achieved with a novel fluorescent probe, NeuO. It enables stable, live neuron imaging in vivo and in vitro across species, thus setting the stage for various neuronal targeting applications including the study of neuron development and degeneration.