Expression of Tobacco Necrosis Virus Open Reading Frames 1 and 2 Is Sufficient for the Replication of Satellite Tobacco Necrosis Virus

Tobacco necrosis virus (TNV) is a small icosahedral plant virus which is often associated with satellite viruses. The genomic RNA of TNV contains six open reading frames (ORFs), of which ORFs 1 and 2 are thought to encode the viral polymerase. We demonstrate that tobacco protoplasts transfected with...

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Veröffentlicht in:	Virology (New York, N.Y.) N.Y.), 1995, Vol.212 (1), p.222-224
Hauptverfasser:	Andriessen, Marco, Meulewaeter, Frank, Cornelissen, Marc
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA-Directed RNA Polymerases - genetics Gene Expression Regulation, Viral Genes, Viral Nicotiana Nicotiana tabacum Open Reading Frames Plant Viruses - genetics Plants, Toxic Satellite Viruses - genetics tobacco necrosis virus Viral Structural Proteins - genetics Virus Replication
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Zusammenfassung:	Tobacco necrosis virus (TNV) is a small icosahedral plant virus which is often associated with satellite viruses. The genomic RNA of TNV contains six open reading frames (ORFs), of which ORFs 1 and 2 are thought to encode the viral polymerase. We demonstrate that tobacco protoplasts transfected with a vector containing TNV ORFs 1 and 2 under the control of the cauliflower mosaic virus 35S promoter, as well as protoplasts derived from transgenic Nicotiana tabacum containing the same gene(s), support replication of satellite tobacco necrosis virus RNA.
ISSN:	0042-6822 1096-0341
DOI:	10.1006/viro.1995.1472