Gene for the Catalytic Subunit of Mouse DNA-Dependent Protein Kinase Maps to the scid Locus

The gene encoding the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) has been proposed recently as a candidate gene for the mouse severe combined immune deficiency (scid) locus. We have used a partial cDNA clone for human DNA-PKcsto map the mouse homologue using a large interspecific backcross panel. We found that the mouse gene for DNA-PKcsdoes not recombine with scid, consistent with the hypothesis that scid is a mutation in the mouse gene for DNA-PKcs.