Genotypic variation and polymorphism of 2S albumins of sunflower

2S albumin fractions from seeds of seven inbred lines and two cultivars of sunflower were compared using a combination of electrophoresis (one and two dimensional) and reversed-phase high performance liquid chromatography. Eleven to thirteen major components were present in each genotype with variat...

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Veröffentlicht in:Euphytica 1995-01, Vol.83 (1), p.15-23
Hauptverfasser: Anisimova, I.N. (Bristol Univ. (United Kingdom). Dept. of Agricultural Sciences), Fido, R.J, Tatham, A.S, Shewry, P.R
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Sprache:eng
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Zusammenfassung:2S albumin fractions from seeds of seven inbred lines and two cultivars of sunflower were compared using a combination of electrophoresis (one and two dimensional) and reversed-phase high performance liquid chromatography. Eleven to thirteen major components were present in each genotype with variation in their proportions and absence or presence. All appeared to be single chain proteins with relative molecular masses (M sub(r)) between about 10,000 and 18,000; some had intra-chain disulphide bonds. Preliminary analyses of single F sub(2) seeds indicated that some components were co-inherited, which could result from their being encoded by linked genes or by a single transcript from one gene.
ISSN:0014-2336
1573-5060
DOI:10.1007/BF01677856