New immobilization method of filamentous cells with thin paper carrier surfaces modified by radiation

A new immobilization method of filamentous cells such as Trichoderman reesei with thin paper carrier surfaces modified by electron beam irradiation has been developed, in which the immobilization was carried out by adhesion of the cells to hydrophobic polymer surfaces. The cells adhered firmly and grew on the surface of the carrier producing the enzyme cellulase. The productivity of the enzyme in immobilized growing cells was higher than that in free cells. The diffusional barrier for substrate, nutrients and products (enzymes) in the immobilized carrier was improved. The effect of the condition of carrier preparation and immunobilization on enzyme productivity was studied to get the optimum condition of immobilization.