Discovery of Bioluminogenic Probes for Aminopeptidase N Imaging

Discovery of Bioluminogenic Probes for Aminopeptidase N Imaging

To find an approach that can image the hydrolysis activity of aminopeptidase N (APN) both in vitro and in vivo, three bioluminescent probes have been well designed and synthesized herein. All of them can be recognized and hydrolyzed by APN to produce bioluminescence emission in the presence of firef...

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Veröffentlicht in:Analytical chemistry (Washington) 2014-03, Vol.86 (5), p.2747-2751
Hauptverfasser: Li, Jing, Chen, Laizhong, Wu, Wenxiao, Zhang, Wei, Ma, Zhao, Cheng, Yanna, Du, Lupei, Li, Minyong
Format: Artikel
Sprache:eng
Schlagworte:
Analytical chemistry
Bioluminescence
Biomedical materials
CD13 Antigens - metabolism
Emission
Enzyme kinetics
Hydrolysis
Imaging
In vivo testing
In vivo tests
Insects
Luminescence
Molecular Probes
Surgical implants
Tissues
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Beschreibung
Zusammenfassung:To find an approach that can image the hydrolysis activity of aminopeptidase N (APN) both in vitro and in vivo, three bioluminescent probes have been well designed and synthesized herein. All of them can be recognized and hydrolyzed by APN to produce bioluminescence emission in the presence of firefly luciferase. To the best of our knowledge, they are the first bioluminescent probes for imaging APN in deep tissues and living animals.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac404176x