PP129. Assessment of oxidative status markers and no bioavailability in hypertensive disorders of pregnancy

Introduction Evidences point that an imbalance between overproduction of reactive oxygen species (ROS) and the lack of antioxidant mechanisms may contribute to endothelial dysfunction observed in hypertensives disorders of pregnancy (HDP), including preeclampsia (PE) and gestational hypertensives (GH). Objectives In the present study, we expanded these previous findings and evaluated systemically the plasma levels of oxidative stress markers (TBARS [2] and carbonyl [3] ) and total antioxidant status (FRAP [1] and reduction of MTT (Medina et al., 2007)), as well as assessed the impact these markers on NO status in healthy pregnant, gestational hypertensive and preeclampsia pregnant. Methods A total of 99 pregnant were enrolled (38 HP, 33 GH and 28 PE. The lipid peroxidation was assessed by (TBARS, [2] ). The determination of carbonyl was performed according to the method of [3] and FRAP assay by Benzie and Strain [1] ). Nitrite levels were evaluated using chemiluminescence assay. Results We found similar values of TBARS among groups, and reduced carbonyl levels in HDP. Conversely, significant increases in plasma activity of antioxidant status of were observed in these groups (GH and PE) compared to healthy pregnant (using both MTT or FRAP method). Conclusion Although antioxidant compensatory mechanism observed in HDP, the levels of nitrite remain reduced in these pregnant compared to healthy pregnant, suggesting that other mechanisms may be involved in the NO bioavailability. This study was funded by the Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG-Brazil), the Conselho Nacional de Desenvolvimento Cientı´fico e Tecnológico (CNPq).