Herbicide-resistant Indica rice plants from IRRI breeding line IR72 after PEG-mediated transformation of protoplasts

The commercially important Indica rice cultivar Oryza sativa cv. IR72 has been transformed using direct gene transfer to protoplasts. PEG-mediated transformation was done with two plasmid constructs containing either a CaMV 35S promoter/HPH chimaeric gene conferring resistance to hygromycin (Hg) or...

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Veröffentlicht in:	Plant molecular biology 1992-11, Vol.20 (4), p.619-629
Hauptverfasser:	Datta, S.K. (Eidgenoessische Technische Hochschule, Zurich (Switzerland). Inst. of Plant Sciences), Datta, K, Soltanifar, N, Donn, G, Potrykus, I
Format:	Artikel
Sprache:	eng
Schlagworte:	Aminobutyrates - pharmacology ANTIBIOTICOS ANTIBIOTICS ANTIBIOTIQUE Biological and medical sciences Biotechnology Blotting, Southern Buffers Cells, Cultured CHEMICAL RESISTANCE Cloning, Molecular Drug Resistance - genetics Fundamental and applied biological sciences. Psychology GENE TRANSFER Genetic engineering Genetic technics GENETIC TRANSFORMATION HERBICIDAS HERBICIDE HERBICIDES Herbicides - pharmacology Hygromycin B - pharmacology Methods. Procedures. Technologies Oryza - embryology Oryza - genetics Oryza - physiology ORYZA SATIVA Phosphotransferases (Alcohol Group Acceptor) Phosphotransferases - genetics Phosphotransferases - metabolism PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Plasmids Polyethylene Glycols PROTOPLASTE PROTOPLASTOS PROTOPLASTS Regeneration RESISTANCE AUX PRODUITS CHIMIQUES RESISTENCIA QUIMICA TRANSFERENCIA DE GENES TRANSFERT DE GENE TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Transformation, Genetic Transgenic animals and transgenic plants TRANSGENIC PLANTS
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Zusammenfassung:	The commercially important Indica rice cultivar Oryza sativa cv. IR72 has been transformed using direct gene transfer to protoplasts. PEG-mediated transformation was done with two plasmid constructs containing either a CaMV 35S promoter/HPH chimaeric gene conferring resistance to hygromycin (Hg) or a CaMV 35S promoter/BAR chimaeric gene conferring resistance to a commercial herbicide (Basta) containing phosphinothricin (PPT). We have obtained so far 92 Hgr and 170 PPTr IR72 plants from protoplasts through selection. 31 Hgr and 70 PPTr plants are being grown in the greenhouse to maturity. Data from Southern analysis and enzyme assays proved that the transgene was stably integrated into the host genome and expressed. Transgenic plants showed complete resistance to high doses of the commercial formulations of PPT.
ISSN:	0167-4412 1573-5028
DOI:	10.1007/bf00046447