Preliminary assessment of the prototype Yfiler® Plus kit in a population study of Northern Italian males

Preliminary assessment of the prototype Yfiler® Plus kit in a population study of Northern Italian males

A total of 150 samples of unrelated males from North of Italy were analyzed with the prototype Yfiler® Plus kit (Thermo Fisher Scientific, Oyster Point, CA). This kit is a short tandem repeat (STR) assay based on six-dye chemistry that amplifies 25 Y-STR loci. Sixteen loci are in common with Yfiler®...

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Veröffentlicht in:International journal of legal medicine 2015-07, Vol.129 (4), p.729-730
Hauptverfasser: Ottaviani, Enrica, Vernarecci, Stefano, Asili, Paola, Agostino, Alessandro, Montagna, Paola
Format: Artikel
Sprache:eng
Schlagworte:
Chromosomes, Human, Y
Diagnostic tests
DNA Fingerprinting - instrumentation
Forensic Medicine
Gene Frequency
Genetics, Population
Haplotypes
Humans
Invoices
Italy
Male
Males
Medical Law
Medicine
Medicine & Public Health
Microsatellite Repeats
Polymerase Chain Reaction - instrumentation
Population Data
Sex crimes
Software
Y chromosomes
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Zusammenfassung:A total of 150 samples of unrelated males from North of Italy were analyzed with the prototype Yfiler® Plus kit (Thermo Fisher Scientific, Oyster Point, CA). This kit is a short tandem repeat (STR) assay based on six-dye chemistry that amplifies 25 Y-STR loci. Sixteen loci are in common with Yfiler® kit and nine are new (DYS576, DYS627, DYS460, DYS518, DYS570, DYS449, DYS481, DYF387S1a/b, DYS533). In this population study, the improvement of adding additional Y-STR markers increased the discriminatory capacity from 0.787 with the minimum haplotype (MHT) loci to 1 with the prototype Yfiler® Plus kit.
ISSN:0937-9827
1437-1596
DOI:10.1007/s00414-014-1078-x