Sensitive and selective magnetoimmunosensing platform for determination of the food allergen Ara h 1
[Display omitted] •First amperometric magnetoimmunosensor for Ara h 1 determination.•Sensitive and selective detection of Ara h 1 in 2h.•LOD of 6.3ngmL−1.•Determinations in food extracts and saliva.•Potential applicability in food safety and consumer protection. A highly sensitive disposable amperom...
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Veröffentlicht in: | Analytica chimica acta 2015-06, Vol.880, p.52-59 |
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Sprache: | eng |
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•First amperometric magnetoimmunosensor for Ara h 1 determination.•Sensitive and selective detection of Ara h 1 in 2h.•LOD of 6.3ngmL−1.•Determinations in food extracts and saliva.•Potential applicability in food safety and consumer protection.
A highly sensitive disposable amperometric immunosensor based on the use of magnetic beads (MBs) is described for determination of Ara h 1, the major peanut allergen, in only 2h. The approach uses a sandwich configuration involving selective capture and biotinylated detector antibodies and carboxylic acid-modified MBs (HOOC-MBs). The MBs bearing the immunoconjugates are captured by a magnet placed under the surface of a disposable screen-printed carbon electrode (SPCE) and the affinity reactions are monitored amperometrically at −0.20V (vs a Ag pseudo-reference electrode) in the presence of hydroquinone (HQ) as electron transfer mediator and upon addition of H2O2 as the enzyme substrate. The developed immunosensor exhibits a wide range of linearity between 20.8 and 1000.0ngmL−1 Ara h 1, a detection limit of 6.3ngmL−1, a great selectivity, a good reproducibility with a RSD of 6.3% for six different immunosensors and a useful lifetime of 25 days. The usefulness of the immunosensor was demonstrated by determining Ara h 1 in different matrices (food extracts and saliva). The results correlated properly with those provided by a commercial ELISA method offering a reliable and promising analytical screening tool in the development of user-friendly devices for on-site determination of Ara h 1. |
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ISSN: | 0003-2670 1873-4324 |
DOI: | 10.1016/j.aca.2015.04.041 |