PARPs and ADP-Ribosylation: 50 Years … and Counting

Over 50 years ago, the discovery of poly(ADP-ribose) (PAR) set a new field of science in motion—the field of poly(ADP-ribosyl) transferases (PARPs) and ADP-ribosylation. The field is still flourishing today. The diversity of biological processes now known to require PARPs and ADP-ribosylation was practically unimaginable even two decades ago. From an initial focus on DNA damage detection and repair in response to genotoxic stresses, the field has expanded to include the regulation of chromatin structure, gene expression, and RNA processing in a wide range of biological systems, including reproduction, development, aging, stem cells, inflammation, metabolism, and cancer. This special focus issue of Molecular Cell includes a collection of three Reviews, three Perspectives, and a SnapShot, which together summarize the current state of the field and suggest where it may be headed. Kraus provides a brief history and overview of the field of poly(ADP-ribosyl) transferases (PARPs) and ADP-ribosylation, as well as an introduction to current areas of research. This piece also includes interviews with two scientists who made ground-breaking discoveries at the dawn of the field: Pierre Chambon and Masanao Miwa.