A highly selective and fast-response fluorescent probe for visualization of enzymatic H2S production in vitro and in living cells

An o-fluorinated-azido-capped rhodamine probe can react with H2S efficiently and selectively to give large off-on fluorescence enhancement. The probe was used to develop an assay for cystathionine β-synthase acitivity and for in situ visualization of endogenously produced H2S in living cells.

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Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2015-07, Vol.51 (52), p.10463-10466
Hauptverfasser:	Wei, Lv, Zhu, Zhentao, Li, Yanyan, Yi, Long, Xi, Zhen
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Survival Cystathionine beta-Synthase - metabolism Fluorescence Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism HEK293 Cells Humans Hydrogen Sulfide - chemistry Hydrogen Sulfide - metabolism Rhodamines - chemistry Rhodamines - metabolism
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Beschreibung
Zusammenfassung:	An o-fluorinated-azido-capped rhodamine probe can react with H2S efficiently and selectively to give large off-on fluorescence enhancement. The probe was used to develop an assay for cystathionine β-synthase acitivity and for in situ visualization of endogenously produced H2S in living cells.
ISSN:	1364-548X
DOI:	10.1039/c5cc03707g