Lensless phase microscopy and diffraction tomography with multi-angle and multi-wavelength illuminations using a LED matrix
We demonstrate lensless quantitative phase microscopy and diffraction tomography based on a compact on-chip platform, using only a CMOS image sensor and a programmable color LED matrix. Based on the multi-wavelength phase retrieval and multi-angle illumination diffraction tomography, this platform o...
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| Veröffentlicht in: | Optics express 2015-06, Vol.23 (11), p.14314-14328 |
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| container_title | Optics express |
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| creator | Zuo, Chao Sun, Jiasong Zhang, Jialin Hu, Yan Chen, Qian |
| description | We demonstrate lensless quantitative phase microscopy and diffraction tomography based on a compact on-chip platform, using only a CMOS image sensor and a programmable color LED matrix. Based on the multi-wavelength phase retrieval and multi-angle illumination diffraction tomography, this platform offers high quality, depth resolved images with a lateral resolution of 3.72μm and an axial resolution of 5μm, across a wide field-of-view of 24mm
. We experimentally demonstrate the success of our method by imaging cheek cells, micro-beads, and fertilized eggs of Parascaris equorum. Such high-throughput and miniaturized imaging device can provide a cost-effective tool for telemedicine applications and point-of-care diagnostics in resource-limited environments. |
| doi_str_mv | 10.1364/OE.23.014314 |
| format | Article |
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. We experimentally demonstrate the success of our method by imaging cheek cells, micro-beads, and fertilized eggs of Parascaris equorum. Such high-throughput and miniaturized imaging device can provide a cost-effective tool for telemedicine applications and point-of-care diagnostics in resource-limited environments.</description><identifier>ISSN: 1094-4087</identifier><identifier>EISSN: 1094-4087</identifier><identifier>DOI: 10.1364/OE.23.014314</identifier><identifier>PMID: 26072796</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Ascaridoidea - cytology ; Cheek ; Humans ; Imaging, Three-Dimensional ; Lenses ; Light ; Lighting - instrumentation ; Microscopy - methods ; Microspheres ; Polymethyl Methacrylate - chemistry ; Refractometry ; Tomography - methods</subject><ispartof>Optics express, 2015-06, Vol.23 (11), p.14314-14328</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-a0b3fee0472ecdbd2724ea505b8c753f19dc165ba56e601bbf45f9a401851d153</citedby><cites>FETCH-LOGICAL-c372t-a0b3fee0472ecdbd2724ea505b8c753f19dc165ba56e601bbf45f9a401851d153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26072796$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zuo, Chao</creatorcontrib><creatorcontrib>Sun, Jiasong</creatorcontrib><creatorcontrib>Zhang, Jialin</creatorcontrib><creatorcontrib>Hu, Yan</creatorcontrib><creatorcontrib>Chen, Qian</creatorcontrib><title>Lensless phase microscopy and diffraction tomography with multi-angle and multi-wavelength illuminations using a LED matrix</title><title>Optics express</title><addtitle>Opt Express</addtitle><description>We demonstrate lensless quantitative phase microscopy and diffraction tomography based on a compact on-chip platform, using only a CMOS image sensor and a programmable color LED matrix. Based on the multi-wavelength phase retrieval and multi-angle illumination diffraction tomography, this platform offers high quality, depth resolved images with a lateral resolution of 3.72μm and an axial resolution of 5μm, across a wide field-of-view of 24mm
. We experimentally demonstrate the success of our method by imaging cheek cells, micro-beads, and fertilized eggs of Parascaris equorum. Such high-throughput and miniaturized imaging device can provide a cost-effective tool for telemedicine applications and point-of-care diagnostics in resource-limited environments.</description><subject>Animals</subject><subject>Ascaridoidea - cytology</subject><subject>Cheek</subject><subject>Humans</subject><subject>Imaging, Three-Dimensional</subject><subject>Lenses</subject><subject>Light</subject><subject>Lighting - instrumentation</subject><subject>Microscopy - methods</subject><subject>Microspheres</subject><subject>Polymethyl Methacrylate - chemistry</subject><subject>Refractometry</subject><subject>Tomography - methods</subject><issn>1094-4087</issn><issn>1094-4087</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpN0D1PwzAQgGELgWgpbMzIIwMpduzEyYhK-JAidYHZcpxLauR8ECeUij9PSgpiOp_0-IYXoUtKlpSF_HadLH22JJQzyo_QnJKYe5xE4vjfe4bOnHsjIxKxOEUzPyTCF3E4R18p1M6Cc7jdKAe4MrprnG7aHVZ1jnNTFJ3SvWlq3DdVU3aq3ezw1vQbXA22N56qSws_dtq36gMs1OUIjLVDZWq1_-3w4ExdYoXT5B5Xqu_M5zk6KZR1cHGYC_T6kLysnrx0_fi8uks9zYTfe4pkrAAgXPig8yz3hc9BBSTIIi0CVtA41zQMMhWEEBKaZQUPilhxQqOA5jRgC3Q93W275n0A18vKOA3WqhqawUkaRjEbK8VipDcT3VdwHRSy7Uylup2kRO5zy3UifSan3CO_OlwesgryP_zbl30DwSt9Mw</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Zuo, Chao</creator><creator>Sun, Jiasong</creator><creator>Zhang, Jialin</creator><creator>Hu, Yan</creator><creator>Chen, Qian</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150601</creationdate><title>Lensless phase microscopy and diffraction tomography with multi-angle and multi-wavelength illuminations using a LED matrix</title><author>Zuo, Chao ; Sun, Jiasong ; Zhang, Jialin ; Hu, Yan ; Chen, Qian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-a0b3fee0472ecdbd2724ea505b8c753f19dc165ba56e601bbf45f9a401851d153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Ascaridoidea - cytology</topic><topic>Cheek</topic><topic>Humans</topic><topic>Imaging, Three-Dimensional</topic><topic>Lenses</topic><topic>Light</topic><topic>Lighting - instrumentation</topic><topic>Microscopy - methods</topic><topic>Microspheres</topic><topic>Polymethyl Methacrylate - chemistry</topic><topic>Refractometry</topic><topic>Tomography - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zuo, Chao</creatorcontrib><creatorcontrib>Sun, Jiasong</creatorcontrib><creatorcontrib>Zhang, Jialin</creatorcontrib><creatorcontrib>Hu, Yan</creatorcontrib><creatorcontrib>Chen, Qian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Optics express</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zuo, Chao</au><au>Sun, Jiasong</au><au>Zhang, Jialin</au><au>Hu, Yan</au><au>Chen, Qian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lensless phase microscopy and diffraction tomography with multi-angle and multi-wavelength illuminations using a LED matrix</atitle><jtitle>Optics express</jtitle><addtitle>Opt Express</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>23</volume><issue>11</issue><spage>14314</spage><epage>14328</epage><pages>14314-14328</pages><issn>1094-4087</issn><eissn>1094-4087</eissn><abstract>We demonstrate lensless quantitative phase microscopy and diffraction tomography based on a compact on-chip platform, using only a CMOS image sensor and a programmable color LED matrix. Based on the multi-wavelength phase retrieval and multi-angle illumination diffraction tomography, this platform offers high quality, depth resolved images with a lateral resolution of 3.72μm and an axial resolution of 5μm, across a wide field-of-view of 24mm
. We experimentally demonstrate the success of our method by imaging cheek cells, micro-beads, and fertilized eggs of Parascaris equorum. Such high-throughput and miniaturized imaging device can provide a cost-effective tool for telemedicine applications and point-of-care diagnostics in resource-limited environments.</abstract><cop>United States</cop><pmid>26072796</pmid><doi>10.1364/OE.23.014314</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Optics express, 2015-06, Vol.23 (11), p.14314-14328 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Ascaridoidea - cytology Cheek Humans Imaging, Three-Dimensional Lenses Light Lighting - instrumentation Microscopy - methods Microspheres Polymethyl Methacrylate - chemistry Refractometry Tomography - methods |
| title | Lensless phase microscopy and diffraction tomography with multi-angle and multi-wavelength illuminations using a LED matrix |
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