Lensless phase microscopy and diffraction tomography with multi-angle and multi-wavelength illuminations using a LED matrix

We demonstrate lensless quantitative phase microscopy and diffraction tomography based on a compact on-chip platform, using only a CMOS image sensor and a programmable color LED matrix. Based on the multi-wavelength phase retrieval and multi-angle illumination diffraction tomography, this platform o...

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Veröffentlicht in:Optics express 2015-06, Vol.23 (11), p.14314-14328
Hauptverfasser: Zuo, Chao, Sun, Jiasong, Zhang, Jialin, Hu, Yan, Chen, Qian
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Sprache:eng
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Zusammenfassung:We demonstrate lensless quantitative phase microscopy and diffraction tomography based on a compact on-chip platform, using only a CMOS image sensor and a programmable color LED matrix. Based on the multi-wavelength phase retrieval and multi-angle illumination diffraction tomography, this platform offers high quality, depth resolved images with a lateral resolution of 3.72μm and an axial resolution of 5μm, across a wide field-of-view of 24mm . We experimentally demonstrate the success of our method by imaging cheek cells, micro-beads, and fertilized eggs of Parascaris equorum. Such high-throughput and miniaturized imaging device can provide a cost-effective tool for telemedicine applications and point-of-care diagnostics in resource-limited environments.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.23.014314