A proteolytic fragment of Trypanosoma cruzi trans-sialidase lacking the carboxyl-terminal domain is active, monomeric, and generates antibodies that inhibit enzymatic activity
trans-Sialidase isolated from trypomastigote forms of Trypanosoma cruzi, the protozoan parasite that causes Chagas' disease, is multimeric and heterogeneous in size. We show here that limited proteolysis of tans-sialidase with papain yields a single monomeric polypeptide chain of 70 kDa that co...
Gespeichert in:
Veröffentlicht in: | The Journal of biological chemistry 1994-03, Vol.269 (11), p.7970-7975 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | trans-Sialidase isolated from trypomastigote forms of Trypanosoma cruzi, the protozoan parasite that causes Chagas' disease,
is multimeric and heterogeneous in size. We show here that limited proteolysis of tans-sialidase with papain yields a single
monomeric polypeptide chain of 70 kDa that conserves full enzymatic activity on soluble and membrane-bound substrates. The
papain fragment lacks most of the 12-amino acid repeats of the carboxyl-terminal domain that comprises about 50% of the native
trans-sialidase. When injected into rabbits, the papain-generated fragment induces antibodies that inhibit trans-sialidase
activity and trypomastigote sialylation. The repeats are also not required for the stability of the enzyme or for the correct
folding during the biosynthesis in Escherichia coli, but seem essential for trans-sialidase oligomerization. We conclude that
trans-sialidase is composed of two structurally and functionally independent domains. |
---|---|
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/S0021-9258(17)37146-6 |